【摘要】 大尺蠖核型多角体病毒(BsNPV)是一个感染茶树害虫的杆状病毒。本实验所用多角体、病毒粒子、DNA都达到较好纯度。在SDS—PAGE照片和扫描图谱上,包涵体蛋白呈现7个多肽带,主要结构多肽的分子量(MW)为27.8k dalton,病毒粒子结构多肽为17种,主要的有两种,MW分别为28.0k和31.3k dalton。BsNPV基因组为环状的dsDNA。用限制性核酸内切酶BamHⅠ,BglⅡ,xhoⅠ,HindⅢ,EcoRI消化,分别产生9,7,10,13和12条带。测出其基因组DNA平均长约为92kb,MW59.6×10~6 dalton。本文着重讨论了杆状病毒包涵体蛋白是由一种多肽还是几种多肽组成的问题。更多还原

【Abstract】 The nuclear polyhedrosis virus infecting tea pests, Buzura suppressuria, is an insect baculovirus (BsNPV), which was isolated only in China A bet-er purity of the polyhedra, virions and DNA used were achieved in this paper Seven polypeptide bands (or peaks) in polyhedral protein and 17 polypeptide bands (or peaks) in virion protein were shown on SDS—PAGE photograph and gel scanning diagram Major structure pclypeptide with a molecular weight of 27.8 k dalton in matrix protein and major structure polypeptide with MW of 28 k and 31.3 k daltons in virion protein have been estimated. The genome of BsNPV is a closed circle double strand DNA. Average size of BsNPV genomic DNA is about 92 kb, which is lower one in insect baculoviruses, after digestion by BamHⅠ(9 bands), BglⅡ(7),XhoⅠ(10), HindⅡ(13), and EcoRⅠ(12). Whether the polyheral protein consists of single or multiply polypeptides is a problem discussed emphatically in present paper更多还原