【摘要】 本文应用盘状电泳和薄层等电聚焦技术对三种革螨——茅舍血厉螨、兵广厉螨和羽腹巨螯螨,作了酯酶同工酶和蛋白质的生化分析,进行了比较研究。结果表明,它们各自的酯酶同工酶酶带和蛋白电泳带都有明显的差别。盘状电泳酯酶同工酶的酶带,茅舍血厉螨有3条,兵广厉螨有4条,羽腹巨螯螨为2条,同时测定了三者酶带的Rf值和相对活性;用等电聚焦电泳分析酯酶同工酶,上述三种螨分别显示11、9、7条亚带,并测定了各亚带的等电点。蛋白质盘状电泳,茅舍血厉螨蛋白带为12条,兵广厉螨为5条,羽腹巨螫螨为5条;在等电聚焦电脉时,上述三种螨分别为24、9、22条蛋白带。更多还原

【Abstract】 A comparative study of the esterase (EST) and proteins among three species of gamasid mites has been carried out by using disc electrophoresis and gel scanning and thin layer gel electrofocusing.The results showed that significant differences were obtained in the RF value of disc electrophoretic zymograms and prominent peaks of densitometric scanning graph and PI of electrofocusing on esterase isozymes and proteins among these three species, which are haemolaelaos casalis(i.e, androlaelaps casalis), cosmolaelaps miles and macrocheles plumiventris. EST isozymes zymograms of disc electrophoresis appeared to be of 3 bands in H.casalis 4 bands in C. miles; and 2 bands in M. plumiventris. Electrophoretic patterns of the whole body proteins were found 12 protein bands in H. casalis, 5 in C. miles and 5 in M. plumiventris. Furthermore 11 subbands in H. casalis, 9 in C. miles and 7 in M. plumiventris has been found by means of EST isozymes electrofocusing. PI of each subband has been measured. A minimun of 24 distinct protein bands was identified from H. casalis, 9 from C.miles and 22 from M. plumiventris by means o[ protein electrofocusing. The preliminary results of proteins patterns may indicate that the H.casalis is more closely related to the C. miles than to the M. plumiventris.The patterns of enzymes and proteins for each species seem highly reproducible. It is suggested that the disc electrophoretic procedures and electrofocusing technique might be employed as an aid for the taxonomic diagnosis, their relationship and gemetics, and the control of their various species belonging to the cohort gamasima as well.更多还原