【摘要】 以0.5%甲醛化空肠弯曲菌(简称空弯菌)菌苗经口免疫昆明小鼠,共两次。于第二次免疫后不同时间段分别处死各组小鼠,取空肠、回肠和结肠中段行冰冻切片,以间接免疫荧光技术检测肠固有膜内的特异性抗体形成细胞,用直接法检测三类(IgA、IgM、IgG)抗体形成细胞。结果提示:甲醛化空弯菌菌苗确能引起较强的肠粘膜免疫应答,固有膜内可产生大量的特异性抗体形成细胞,尤以第12天～15天为甚,且以IgA类抗体形成细胞的升高为主,IgG及IgM类抗体形成细胞亦有相应的增加。更多还原

【Abstract】 Mice, the Kunming strain, were immunized by orogastric intubation with 0.5% formalized Campylobacter jeiuni-Suzhou 131(CJ-S131)bacteria (two times at an interval of seven days). Each group wasat random sacrificed at differant stages after the second immunization. The frozen tissue sections were taken from the middle of the jejunum, ileum and colon, respectively. Specific antibody-containing cells(ACC) in intestinal mucosa were examined by indirect immunofluorescent technique and three kinds (IgA,IgM,IgG) ACC were examined by the direct one. The results have shown that intestinal mucosa immunity can be strongly induced by the formalized CJ-S131 vaccine. There are a large number of specific ACC in the lamina propria, particularly from the 12th to 15th days. However, IgA type of ACC is predominant, IgG and IgM type ACC can increase relatively.更多还原