【摘要】 本文用高分辨400MHz NMR’H谱对中华眼镜蛇细胞膜毒素CT-Ⅰ和CT-Ⅱ组分进行了比较研究,包括COSY谱,T₁和T₂驰豫测量.通过分析比较对CT-Ⅰ和CT-Ⅱ芳环质子的共振峰进行了初步识别.T₁和T₂驰豫测量结果表明有较大T₁和T₂值的Phe 10和Tyr 11位于伸展的有较大构象运动的分子表面区域,其余芳香残基则“埋藏”于分子内部.更多还原

【Abstract】 Cytotoxin CT-Ⅰ and CT-Ⅱ from the venom of Chinese cobra (Naja Naja Atra) were investigated by high-resolution NMR techniques at 400 MHz, including 2D-COSY spectrum,T1 and T2 measurament.The aromatic 1H NMR resonances were assigned.Phe10 and Tyr11 which have longer T1 and T2 than other aromatic residues is in a region of the macromolecule that is extended and confor-mationally mobile.The others are located in a well-organized environment.更多还原