【摘要】 用电融合法进行了萝卜胞质雄性不育甘蓝(Brassica oleracea var.capitata)叶肉原生质体与大白菜(Brassica campestris var.pekinensis)悬浮细胞原生质体的融合.使用宽间距(电极间距1.5mm)电融合小室,操作简易.两种原生质体的异源融合率最高可达46±6%,其中异源双体融合率可达11±1%.异核体在与双亲原生质体共培养(10~4/ml)时,24小时就有近10%发生第一次分裂,48小时后可达70%左右.培养24—48小时后用稀释法挑选出异核体,部份异核体可在低密度(10~2/ml)甚至单细胞条件下持续分裂.由此获得了异核体来源的愈伤组织,并在分化培养基上再生出了根.更多还原

【Abstract】 Electrofusion was performed between mesophyll protoplasts of cabbage (Brassica oleracea var. capitata, with Raphanus ’Ogura’ cytoplasmic male sterility) and suspension protoplasts of Chinese cabbage(B. Campestris var. pekinensis). With a wide-interval(1.5 mm between two electrodes) electrofusion chamber,the operation is simple and the highest ratio of heterofusion up to 46±6% could be obtained, among which the percentage of di-heterofusion was 11±1%.When heterokaryons were cultured with unfused protoplasts at a density of 104/ml,about ten percent of heterokaryons resulted in first division within 24 h. Division ratio of heterokaryons reached 70% after 48 h in culture. Heterokaryons having chloroplasts from mesophyll of cabbage and cytoplasm from suspension of Chinese cabbage were isolated manually by dilution of the culture at the first or second day. Some of them undergone sustained division at a density as low as 102/ml or even in a single heterokaryon culture.The calli derived from such a way regenerated roots on a differentiation medium.更多还原