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生物系统超弱发光的探测:绿豆、大鼠血液和3T3细胞的发光
DETECTION OF ULTRA-WEAK PHOTON EMISSION FROM BIOLOGICAL SYSTEMS: LOW-LEVEL CHEMILUMINESCENCE OF MUNG BEAN SEEDLING, RAT BLOOD AND 3T3 CELLS
【摘要】 本文报道一台用于生物系统微弱发光研究的高灵敏单光子计数系统、能在200—900nm范围测量生物样品的发光强度、光谱和发光动力学.由于光电倍增管以液氮冷却、噪声降至40cps,在99.9%置信度和6小时测量条件下,最小可探测0.3光子/秒、厘米~2的微弱光子流.用该系统研究了萌发绿豆、大鼠血液和体外培养正常和转化3T3细胞的发光.这些样品的发光都含有一个光诱导成份,且以不同的速率衰减、最后达到稳定水平代表了这些生物系统自发的代谢发光.实验发现,转化的3T3细胞的发光强度比正常细胞约低30%.
【Abstract】 A highly sensitive single-photon counting system for detecting ultra-weak photon emission of biological systems has been described. It could measure the intensity, spectrum and kinetics of photon emission from biological samples in the wavelength range of 200-900 nm. Liquid nitrogen was used to cool photo-multiplier, whith reduced the noise down to 40 cps. With this equipment a photon current density of 0.3 photons.s-1昪m-2could be detected at a significance level of 99.9% within 6 h.The low-level luminescence of mung bean seedlings, rat blood, normal and transformed 3T3 cells in vitro were measured with this system. It was found that a photo-induced component existed in the luminescence for the all samples studied and decayed at a rate different from each other until a steady level was reached, the later could represent the metabolic chemiluminescence of the samples. It was also noted that the luminescence intensity of transformed 3T3 cells was 30% lower than that of normal cells.
- 【文献出处】 生物物理学报 ,Acta Biophysica Sinica , 编辑部邮箱 ,1988年02期
- 【被引频次】45
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