【摘要】 研究了螺旋藻藻胆体的吸收光谱,室温和液氮温度荧光发射光谱和激发光谱.完整藻胆体的室温荧光峰位于678nm,不完整藻胆体位于672nm.在完整藻胆体的液氮温度荧光光谱中只有一个发射峰,不完整藻胆作有两个峰.研究结果表明C-藻蓝蛋白与别藻蓝蛋白之间的连接和别藻蓝蛋白与别藻蓝蛋白-B之间的连接具有不同的稳定性;前者稳定性较差,易解离.对藻胆体内藻胆蛋白之间的光能传递进行了讨论.更多还原

【Abstract】 Absorption spectra, fluorescence emission spectra and excitation spectra of phycobilisomes at room temperature and liquid nitrogen temperature were studied. The room temperature fluorescence emission maximum of intact phycobilisomes was located at 678 nm, that of non-intact phycobilisomes was at 672 nm. There was one peak in liquid nitrogen temperature fluorescence emission spectrum of intact phycobilisomes, and two peaks in that of non-intact phycobilisomes. Our research results indicate that the linkages between C-PC and APC, and APC and APC-B have different stabilities, probably the linkage between C-Pc and APC is easy to dissociate.The light energy transfer among phycobiliproteins in phycobilisomes has been discussed.更多还原