【摘要】 用蔗糖梯度离心法对Novikoff肝癌细胞的多聚（A）⁺mRNA进行了链长分部。沉降在13S和15S的组分6和组分7富集了B₂₃mRNA,其体外转译产物可特异地被抗蛋白B₂₃的抗体免疫吸附,被吸附的蛋白在SDS-PAGE中迁移到大约37,000道尔顿的区带。另外,用多聚核糖体免疫吸附技术提纯了少量B₂₃mRNA,它在体外转译系统中也指导合成了分子量约为37,000道尔顿的蛋白质。更多还原

【Abstract】 Poly(A)+mRNA from Novikoff hepatoma cells were size-fractionated by sucrose gradient centrifugation. The RNA in fraction 6 and 7 sedimenting at 13S-15S was enriched in a specific mRNA, whose cell-free translation products can be immuno-adsorbed by the antibody to nucleolar protein B23 and migrated to the position of approximately 37 Kd on a SDS-polyacrylamide gel. Additionally, polysome immunoadsorption technique was used to partially purify a small amount of specific mRNA which directed in vitro synthesis of a specific protein, also having a -molecular weight of about 37 Kd.更多还原