【摘要】 我们以前的工作指出,生长抑素可以预防链佐霉素对大鼠胰岛B细胞的损伤。本工作测定给药前后大鼠血清和组织中Zn、Cu含量,以进一步观察链佐霉素破坏大鼠胰岛B细胞后生长抑素预防效应的可能机制。结果为:链佐霉素(STZ)(35mg/kg,ip)注射后24h的大鼠,血清Zn浓度下降,Cu浓度升高,Zn/Cu比值倒置;用生长抑素作预防性注射后,血清Zn浓度与正常对照水平相同,Cu浓度虽稍有升高,但Zn/Cu比值正常。保护组的血清Zn与损伤组比较,差异极为显著。两组的Zn/Cu比值同样也具有显著差异。在给药后72h,血清Zn/Cu比值在各组之间仍呈上述关系,但差异减小。在大鼠胰腺,注射链佐霉素后胰腺组织Zn浓度未见改变,但生长抑素作预防性注射可使组织锌含量增加。上述结果提示,在生长抑素对胰岛B细胞的保护机制中可能有Zn和Cu的参与。预防性注射生长抑素所引起的高Zn状态可能有利于机体细胞含Zn酶类的活性,增强已损伤细胞的修复。更多还原

【Abstract】 Our previous work suggested that somatostatin might have preventive effecton the islet B cell injury induced by streptozotocin. In present paper the zinc andcopper contents in both serum and pancreas in rats after administrations of eitherstreptozotocin or somatostatin plus streptozotocin were examined to analyse thepossible mechanism underlying the effect of somatostatin. The results were asfollows: The zinc concentration in serum was reduced 24 or 72 hours after strepto-zotocin (35 mg/kg, ip), whereas the copper concentration in serum was increasedunder the same conditions. The ratio of Zn/Cu in serum was thus reversed ascompared with that of normal rats. These changes could be prevented whensomatostatin (30 μg/kg, sc) was injected subcutaneously 10 minutes before strep-tozotocin. No change of zinc concentration in pancreas was observed whenpretreated with streptozotocin, but a significant increase of pancreatic zinc wasfound in rats pretreated with both somatostatin and streptozotocin. These datasuggest that zinc and copper may be involved in the preventive mechanism of soma-tostatin against the islet B cells damage induced by streptozotocin. The normalserum level and higher pancreatic level of zinc content after somatostatin may bebeneficial to the activity of Zn-included enzymes and the repair of injured cells.更多还原