【摘要】 本文选用PMSG-hCG预处理的未成年雌性大鼠卵巢黄体经DNA酶-胶原酶消化后,制成黄体细胞悬浮液。在黄体细胞悬浮液的培养管内,加入hCG100mIU/ml 100μl,同时加入三种浓度（0.02,0.2,2mmol/L）的二十一种氨基酸,充入95％O₂+5％CO₂,37℃孵育1.5h,用放射免疫分析方法测孕酮含量。结果表明:二十一种氨基酸中,只有酪氨酸、苏氨酸、丝氨酸可对抗hCG致孕酮生成作用。这三种氨基酸在结构上均具有羟基,都是蛋白质磷酸化的位点,据此推测:这三种氨基酸抗hCG致孕酮生成作用可能与干扰蛋白激酶的磷酸化有关。更多还原

【Abstract】 Corpus luteum cells from immature female rats pretreated by PMSG-hCGwere digested with DNAase-collegnase. After centrifugation corpus luteum cells.were suspended in EDTA solution. The effect of 21 amino acids on the hCG-induced progesterone production was compared. Three dose levels (0.02 mol/L,0.2 mol/L and 2 mol/L) of each amino acids were tested and the dose of hCGwas 100 mIU/ml. Each tube containing 60 million cells/0.5 ml, hCG, anddifferent amounts of the amino acids as desired was incubated at 37℃ for1.5 h. The change of progesterone in the tube was measured by RIA. Of the twenty-one kinds of amino acids, only tyrosine, threonine and serineinhibit the progesterone production induced by hCG. Since these three aminoacids all have hydroxyl group for phosphorylation, it is suggested that the inhibi-tory effect of the three amino acids on progesterone production induced by hCGmay be due to the interference of protein kinase phosphorylation.更多还原