【摘要】 用Maximow双盖片法对新生B₆C₃鼠的小脑进行总数达160多片的组织—器官型培养,在普通光镜及明相差显微镜下对髓鞘的生长过程作动态观察的分析。发现在培养10—14天开始有髓鞘形成,至培养16天已明显呈现,并可维持到一个月或更久。结合改良Holmes银染及扫描电镜、透射电镜的观察,为髓鞘体外观察提供模型。作者分析了影响髓鞘体外培养的各种因素,其中培养液内血清的质与量均有密切关系。从而为在国内具体条件下取得成功培养提供参考。更多还原

【Abstract】 Myelinating cultures of more than 30 newborn mouse cerebelli were successfully taken with Maximow’s double-coverslip technique. Living observations were made by both ordinary light and bright phase contrast microscopy. A modified Holmes’ silver stain was also used. It is presumed that there are many factors affecting myelination in vitro, especially for the serum which might play an important role in the conditioned medium, and these were ever discussed. Our experiences in performing a successful cerebellar culture in early postnatal vertebrates might serve as a useful model and provide a multidiscipline strategy in the field of myelinati0n and demyelination study of the modern neuroscience,更多还原