【摘要】 用半微量法分析了N-亚硝基-N-甲基脲素诱发的大鼠乳腺癌（含有Ha-ras-1癌基因）的神经节苷脂谱及其唾液酸转移酶活性。与正常大鼠乳腺组织比较,该乳腺癌组织的神经节苷脂谱有明显改变,并伴有GD₃合成酶活性的增高。作者对可能引起这一改变的乳腺癌基因背景进行了讨论。更多还原

【Abstract】 Ganglioside patterns and sialyltransferase activities of NMU induced rat mammary carcinoma, which contains Ha-ras-1 oneogenes, have been investigated. The total ganglioside content in the tumor tissue (21.3μg/g wet weight) was significantly higher as compared with normal tissue (12. 2μg/g wet weight). GD1b, GT1b and GQ1b were absent in normal tissue but occurred in tumor tissue, and sepecially GT1b which rose to compose 12.2% of the total gangliosides. GD3 synthetase activity was increased in rat mammary carcinoma, but the GM3 synthetase activity remained normal. This leads to the change of the ganglioside pattern in tumor tissue. Results indicate that genetic changes of tissue might be responsible change in its ganglioside composition and changes in sialyltransferase activity, and the ganglioside change in transformed tissue might be a consequence of transforming gene activation.更多还原