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人体血清中维生素A的荧光测定(微量法)

FLUOROSPECTROMETRY OF VITAMIN A IN HUMAN SERUM (MICROANALYSIS)

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【作者】 庄小陶; 陆瑞芳;

【Author】 Zhuang Xiaotao, Lu Ruifang(Department of Nutrition and Food Hygiene, School of Public Health, Shanghai Medical University, Shanghai)

【机构】 上海医科大学公共卫生学院营养与食品卫生学教研室; 上海医科大学公共卫生学院营养与食品卫生学教研室;

【摘要】 使用荧光分光法测定人体血清中维生素A灵敏、精确、取血量很少、适合大规模人群营养调查。本法与三氟醋酸比色法比较,结果满意,相关显著。本文对此方法的精密度、回收率及其他实验条件作了探讨,并调查了本市180名健康成人血清中维生素A值。

【Abstract】 The use of flurospectrometry to determine vitamin A in human serum is shown to be sensitive, accurate and needs a lesser amount of sample. It is suitable for nutritional surveys of large populations. This method favorably with the trifluoro-acetic acid oolorimetric method with a significant correlation coefficient.Experimental conditions of this method, its accuracy, sensitivity and recovery are discussed. Moreover, vitamin A contents in sera of 180 healthy adults in Shanghai has also been determined by this method.

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