【摘要】 用HeLa和Vero细胞检测了30株来源不同的空肠弯曲菌和结肠弯曲菌的细胞毒素。其中17株细菌来自急性腹泻患儿的粪便,11株来自家禽或家畜,2株来自正常健康儿童。在被检测的30株细菌中有19株产生细胞毒素,其中13株从急性腹泻患儿粪便分离,6株来自家禽或家畜。来自急性腹泻患儿菌株引起HeLa和Vero细胞半数组织培养出现细胞病变量(50％tissue culture cyfopafhologic dosc,TCD₅₀的最高稀释度（±SD）分别为:2^-2.0856±2^-2.9426,2^-1.2742±2^-2.1457;而从家禽分离的菌株对2种细胞的TCD₅₀分别为2₍1.9412±2^-2.4876,2^-1.7270±2^-2.6910。我们还发现当细菌在布氏肉汤中培养72h时产毒量高于培养24、48、96h。更多还原

【Abstract】 Thirty strains of Campylobacter jejuni and Campylobaoter coli were assayed by HeLa and Vero cells for cytotoxins. 17 of these 30 strains were isolated from feces of children with acute diarrhea, 11 from domestic animals and 2 from healthy children, 19 were found to be toxin-producing strains. Among these 19 strains, 13 were isolated from children with acute diarrhea, and 6 from domestic animals. The maximum dilution (x±SD) of ultrafiltrations of C. jejuni and C. coli from children with diarrhea and those from domestic animals causing 50 % cytopathologio changes in HeLa and Vero cell assays were 2-2.085±2-2.9426, 2-1.27452±2-2.1457;and 2-1.9412± 2-2.4876, 2-1.7270±2-2.6910, respectively. The titres of cytotoxins produced by C jejuni and C. coli in Brucella culture broth after 72hours were found to be higher than those after 24, 48, or 96hours.更多还原