【摘要】 本文报道检测鸭乙型肝炎表面抗原(DHBsAg)及抗体的斑点酶免疫测定法(Dot Euzyme Immunoassay,Dot EIA)。以硝基纤维素膜为载体依次加被检血清、抗DHBV免疫血清、辣根过氧化物酶标记葡萄球菌A蛋白(SPA-酶结合物),可测标本中DHBsAg。依次加纯化DHBV、被检血清、SPA-酶结合物则可测血清中抗体及效价。用此法检测自然感染鸭血清40份,实验感染鸭血清220份,并与DHBV核酸斑点杂交法比较分析,两种方法符合率94.2％。用本方法测定本室制备的兔抗DHBV免疫血清,抗体效阶为1/3200。Dot EIA简单、快速、敏感、特异,是研究DHBV较有实用价值的一种方法。更多还原

【Abstract】 A dot enzyme immunoassay (dot EIA) for detecting DHBsAg and anti-DHBs is reported. Duck sera for assay of DHBsAg were spotted onto nitrocellulose filters dried and blocked. The filter membrane was then soaked in rabbit anti-DHBV serum, washed and incubated with peroxidase labeled staphylococcus protein A, followed by addition of 4-chloro-1-naphthol substrate. DHBsAg positive sera showed purple spots on the filter. "When purified DHBV were spotted, this test could be used to assay anti-DHBV. Compared to the DHBV DNA hybridization test among 260 sorum samples, the percentage of coincidence between the EIA and DHBV DNA hybridization tests was 94.2%. Rabbit anti-DHBV prepared in our lab showed an assay titer of 1:3 200. Dot EIA is not only rapid and simple but also specific and sensitive. It will be of practical use in lab for screening large numbers of duck serum samples without the need of sophisticated equipment.更多还原