【摘要】 报道甲地孕酮酶标免疫测定和兔血药浓度测定。抗血清特异性:除与甲孕酮、雌三醇交叉反应分别为29.6％和1.9％外,其余5种甾体均小于0.1％。灵敏度为0.1ng;回收率:0.5、1和2ng分别为99.5、80和86.8％;批内CV<10％,批间CV<20％。静脉注射和口服后兔血浆浓度分别是160和62ng/mL。静脉注射和口服后T1/2分别为15±3h和14±4h。生物利用度为64％。虽然酶标免疫测定(Enzyme Immunoassay),EIA)的灵敏度不及放射免疫测定(RIA),但本法无同位素污染,不需特殊设备和专门实验室,适合于基层医疗单位使用。更多还原

【Abstract】 This paper reports an enzyme immunoassay(EIA) method for measuring plasma megestrol aoetate(MA). The antiserum exhibited no significant oross-reaotion with various steroids tested except medroxyprogesterone 29.6% and estroiol 1.9%. The sensitivity of the assay is 0.1ng. The recovery rates of 0.5, 1 and 2ng were 99.5, 80 and 86.8% respectively. The C.V. were<10% within run and <20% between days. The plasma MA concentration after iv administration was 160ng/ml which was about 2 to 8 fold that after the oral route (62ng/ml). T 1/2 iv and T1/2 p.o. were 15 ±3 and 14 ±4 h respectively, and bioavailability (F) was 64%.更多还原