【摘要】 报道以谷氨酸棒状杆菌(Corynebacterium glutamicum)No.11为试验菌进行L-脯氨酸的生物合成。在条件研究的基础上,通过以外源方式导入L-脯氨酸的直接前体L-谷氨酸来提高产量,并进一步研究了在适当时候,在相关物乙醇浓度为1.5％的协同条件下,同时引入谷氨酸钠浓度为3.0％,此时L-脯氨酸产量比对照组提高20％。此外,还观察了醇醛类物质和天冬氨酸对本试验菌的L-脯氨酸生物合成的影响。更多还原

【Abstract】 The purpose of this study was to increase the biosynthetic production of L-proline by the Corynebacterium glutamicum No. 11 through the addition of its extra-direct precursor L-glutamic acid. It was found that at a suitable time, the simultaneous addition of 1.5% ethanol and 3.0% sodium glutamate increased the yield by 20%.Furthermore, the effects of adding ethanol, aldehyde and aspartic acid were also observed.更多还原