【摘要】 用香烟主流烟雾颗粒物质(TPM),即香烟焦油(Tar)作受试物,用不同放射比活度的~3H-TdR和不同浓度的羟基脲观察TPM对人末梢血淋巴细胞存活率和非程序DNA合成(UDS)的影响。结果表明,TPM能使~3H-TdR的掺入明显增加,10～100μg/ml范围内呈剂量-反应关系。本文还对人外周血淋巴细胞UDS液闪计数的试验方法进行了探讨。更多还原

【Abstract】 Mainstream cigarette smoke total particulate matter(TPM)wascollected by means of a smoking machine,then it was extracted by sonication with acetone,and the extracts were solvent-exchanged to dimethl sul-foxide for experiments.TPM was tested for cell survival and UnscheduledDNA synthesis(UDS)in human lymphocytes with different concentrationsof hydroxyurea and different specific activities of[~3H]-Thymidine.Theresults suggested the scintillometric method used for dctection of UDS wassufficiently sensitive to be used as a tool for genetic risk assessment ofmainstream cigarette smoke particles.更多还原