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猪瘟抗体检测方法的研究

SEROLOGICAL TESTS IN DETECTION OF ANTI-HOG CHOLERA ANTIBODIES

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【作者】 吴明杰; 杜念兴; 徐为燕;

【Author】 Wu Mingjie, Du Nianxing and Xu Weiyan(Section of Veterinary Microbiology, Nanjing Agricultural University)

【机构】 南京农业大学兽医微生物教研组; 南京农业大学兽医微生物教研组;

【摘要】 本文用间接血凝试验、酶标SPA酶联免疫吸附试验、生物素标记SPA酶联免疫吸附试验以及兔体中和试验进行检测猪瘟抗体比较,结果表明它们之间有良好的相关性。间接血凝试验操作简单、易推广。生物素标记葡萄球菌蛋白A介导的酶联免疲吸附试验是新建立的血清学方法,敏感性高,非特异性低,阴、阳分界线可定在1:10。

【Abstract】 Serological tests in detection of anti-hog cholera antibodies were investigated. Indirect hemagglutination test, SPA-ELISA, biotin labeled SPA-ELISA and neutralization test in rabbits were compared. The resuli indicated that there was well correlation among them. Indirect hemagglu-tiantion test was simple to operate and worthy to be popularized. Biotin labeled SPA-ELISA, a recently developed test, was high in sensitivity and low in non-specific reaction, and the demarcation line between positive and negative could be located at 1:10.

  • 【文献出处】 南京农业大学学报 ,Journal of Nanjing Agricultural University , 编辑部邮箱 ,1988年S1期
  • 【被引频次】1
  • 【下载频次】103
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