【摘要】 本文就杨尺蠖核多角体病毒的几种回收方法进行了比较。回收试验表明,以丙酮—乳糖（7.4—8.4％）共沉法回收 AciNPV 的多角体（PIB）是可行的。制备物的最终含量为 4.96×10¹⁰PIB/g,回收率平均达 80.5％。生物活性测定证明,回收的新鲜制备物,对3龄初幼虫的致病力是很好的。其 LC₅₀ 及其95％的置信限分别为2.84×10³PIB/ml和 1.70×10³—4.96×10³PIB/ml。 使用该程序,可省去昂贵的离心设备,在实验室一般设备的情况下,一次上样1500—3000 g病死虫,仅需 3小时即可获得最终产品。配制的病毒制剂,可用于防治杨尺蠖 30—65ha。大批量生产证明,该流程操作简便,省工省时,一次上样量较大,完全可以替代离心法,回收的成本也很便宜。更多还原

【Abstract】 Several methods for recovery procedure of the polyhedral inclusion bodies of nuclear polyhedrosis virus of the poplar looper, Apocheima cinerarius, were compared. Recovery tests indicated that the PIB of AciNPV were precipitated from the concentrated suspensions in 7.4-8.4% solutions of lactose by adding acetone. Counts of the final preparation showed 4.96×1010 PIB/g. Yields of 80.5% were obtained. Bioassay tests indicated that the pathogenicity of the fresh preparations recovered was very good for the early third-instar larvae. The LC50 and 95% fiducial limits acre 2.84×103 PIB/ml and 1.70×103-4.96×103 PIB/ml respectively.In this procedure, costly centrifugal equipment was omitted, 1500-3000g diseased larvae could be reduced to final production in 3hr with laboratory general equipment. In this case, the virus formulation prepared may be used to control the poplar looper larvae in about 30-65 ha in the forest. Mass production has been proved to be a useful replacement for the centrifugation method, and the cost of the recovery virus is cheap.更多还原