【摘要】 为了生产病毒制剂提供大量病原物,本文就影响AciNPV增殖的因素及林间复制技术进行了研究。采用浸叶法接毒感染2—4龄初期幼虫,以便寻找适宜的接毒浓度、虫龄与温度范围。试验证明,接毒的适宜虫龄以4龄初期为最好;接毒浓度以1.1×10⁶-1.1×10⁷PIB/ml之间为宜,平均幼虫多角体产量为2.42—2.64×10⁹PIB/头;增殖的适宜温度在22—27℃之间,温度过高对病毒的增殖不利,为在35℃下增殖则受到抑制。在林间复制时,其喷洒剂量以2.5×10¹⁰PIB/亩宜,虫龄以2—3龄占85％左右为好;3—4龄幼虫占85％时,应加大用量1倍。更多还原

【Abstract】 This paper deals with results of our investigation on the factors affe-cting the propagation of the nuclear polyhedrosis virus （NPV） of the polarlooper,Apocheima cinerarius Erschff. The concentration had a conspicuous effect on the propagation of thenuclear polyhedrosis virus.Among the concentration tested,the 1.1×10⁶-1.1×10⁷ PIB/ml was found to be the best on early four instars aver-age 2.42-2.64×10⁹ PIBs/larvae.When the larvae of poplar looper werefed with the poplar leaves contaminated with polyhedral inclusion bodies（PIB） of the virus,the mortality rate of the infected larvae was depen-ded on concentration of the virus suspension and laral instar. The amount of propagated NPV was found to be proportional to theconcentration of the inoculum suspension.The lag of the death of larvaeinoculated with NPV was influences by the temperature and suspensionconcentration of the polyhedral virus.The propagation of the virus wasinhibited at 35℃ and the optimal temperature was 22—27℃.The NPV would propagaten better in early four-instar larvae than in other instars onan average of 2.42×10⁹ PIBs/larvae. The propagation of field indicated that optimum suspension contrationwas 2.5×10⁶ PIBs/mu to second-instars and third-instars.Dry proparationof PIB of nuclear polyhedrosis virus of Apocheima cinerarius by precipit-ation with acetone from lactose suspensions indicated that the LC₅0 val-ues of 2.84×10³ PIBs/ml for early third-instar larvae and 95% fiduciallimit was 1.70×10³-4.96×10³ PIBs/ml.更多还原