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体外培养乳大鼠心肌细胞的电镜观察
ELECTRON MICROSCOPIC OBSERVAION ON IN VITRO MYOCARDIAL CELLS OF NEONATAL RATS
【摘要】 借助透射电镜观察了培养乳大鼠心肌细胞的超微结构。培养3—4天时,线粒体丰富,嵴排列稀疏且不规则,粗面内质网发育中等,自噬小泡易见,但未见明显的肌原纤维,偶有散布的肌丝;培养6—7天时,线粒体更丰富,体积增大,嵴密集平行排列,肌原纤维散在分布或相互平行,偶见分支连接,肌节结构典型,Z线较宽,闰盘位于Z线平面,偶见橫小管。本文还对上述形态学变化进行了讨论。
【Abstract】 The ultrastructure of cultured myocardial cells of neonatal rats was investigated using the electro-microscope. After the cells were incubated for three to four days, it was found that the crests of mitochondria were Ioosely and irregularly arranged, the granular endoplasmic reticula developed, and the autophagic vascuoles were apparent, but myofibrils could not be appreciably observed, although discrete myofilaments were visible. Having been incubated for six to seven days, the mitochondria became larger and more abundant, with their crests arranged in a dense and parallel fashion, or occasionally branched out and connected one another, Such myobrils contained sarcomeres with thickened Z lines, at the level of which the intercalated disk were located, as well as sparsely distributed transverse tubules. Finally, the morphologic changes are dicussed.
- 【文献出处】 泸州医学院学报 ,Journal of Luzhou Medical College , 编辑部邮箱 ,1988年01期
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