【摘要】 <正> 红细胞的谷胱甘肽还原酶在有还原型辅酶Ⅱ存在下可催化氧化型谷胱甘肽(GSSG)还原为还原型谷胱甘肽(GSH),NADPH+GSSG?NADP+GSH。该酶的辅基为黄素腺嘌呤二核苷酸(FAD),因此,当核黄素缺乏而导致FAD不足时,则可使此酶的活性下降。通过测定红细胞中的谷胱甘肽还原酶活性系数(GRAC)来评价机体核黄素的营养状况,更多还原

【Abstract】 Through comparing the conditions for determining blood glutathione re-ductase （BGR） activation coefficients （AC）, it is proved that using saponinsolution to prepare blood hemolysate is better than using distilled water, andthat 5, 5’ -dithio-bis-2-nitrobenzoic acid produced by ROTH Company is bet-ter than Shanghai in developing。 It is also proved that HPO₃ solution added with EDTA and NaCl is bet-ter than HPO₃ solution alone, and that after enzyme reaction, tube A addedwith FAD or not has no effect on AC. Using improved methods, 111 peasants are determined. O.D of tube A is0.07±0.02 and O.D of tube B is 0.09±0.02. The results of 64 peasants examination demonstrated that the correlationof AC and 4 hour excretion of riboflavin in urine after an oral dose of 5mgriboflavin is negative. AC evaluating criteria corresponding to excretion of ribo-flavin in urine is: normal<1.25; moderate: 1.25-1.65; inadequate: 1.65-2.00; lack:>2.00.更多还原