【摘要】 本文对缺血组织进行了水溶性莤素红(Alizarin Red S以下简称ARS)钙组织化学染色观察。此外还作了HE染色和心电图(ECG)检查。ARS染色阳性说明细胞内钙浓度异常增高。结果:正常对照组和缺血损伤组非缺血区对照ARS染色均为阴性;缺血区中,15分钟组9例动物中的1例和45分钟组12例动物中的7例为ARS阳性。上述各组的系列切片HE染色均未见坏死迹象。ECG检查发现8只动物有坏死性心电改变,出现异常Q波或QS波,恰与ARS(+)动物呈严格的对应关系。这些结果证明,ARS染色是一项敏感可靠的判定不可逆性缺血再灌注损伤的指标,它能够及时在细胞原位反映出钙增多这一致死性代谢改变。更多还原

【Abstract】 Ischemic and post-ischemic injury was made by 45 minute reperfusion after 15 and 45 minute myocardial ischemia with blocking and reopening the left ventricular branch of coronary artery in vivo in 21 rabbits. Sections from both ischemic and non-ischemic areas were stained with Alizarin Red S (ARS) since calcium accumulation in cytoplasms and mitochondria was a mark of irreversible ischemic damage. Examinations of HE staining and electrocardiogram (ECG) were also performed.更多还原