【摘要】 我们与我校第三医院妇产科合作,于1987年进行了6例精、卵输卵管内移植术。有2例妊娠,成功率为33％（6例应试者的年龄均在41岁以下,有近10年不孕史,但其输卵管至少一侧是通畅的）。应用Ham F₁₀培养液,加脐血清或母血清配成卵泡冲洗液（FM）、精子洗液（SW）、生长液（GM）和移植液（TM）。用克罗米芬（CC）和人绝经期促性腺激素（HMG）绣发超排卵。当超声波探测有2个卵泡直径达16 mm时,肌内注射人绒毛膜促性腺激素（HCG）,32～34小时后开腹取卵,在实体显微镜下收集卵冠丘复合体（OCCCs）,并对其做显微加工。在取卵前2～3小时,令被试者的丈夫供新鲜精液。待精液液化后离心2次,去掉精浆,加入SW液,再将精液放入CO₂箱中孵育。用特制的Catheter移植管吸取2个OCCCs和25μl精液,注入到一侧输卵管内。以同样方式给对侧输卵管注入精卵。术后11天测定B-HCG以确诊妊娠。更多还原

【Abstract】 6 cases of gamete intra-fallopian transfer （GIFT） were performed in cooperation with the Department of Gynaecology and Obstetrics of The Third Teaching Hospital of our university in 1987. All the six women aged less than 41 years and have failed to be pregnant as long as ten years. One fallopian tube is patent at least. Two of them were pregnant after operation of GIFT. The rate of success was 33％. We used Ham’s F10 medium mixed with different concentrations of fetal cord serum or mother’s serum to prepare respectively follicular flushing medium （FM）, sperm washing medium （SW）, growth medium （GM） and transfer medium （TM）. Clomiphene citrate and human menopausal gonadotropin （HMG） were used for inducing growth of follicle. HCG was given by intramuscular injection when two or more follicles were measured 16 mm in diameter by pelvic ultrasound. 32-34 hours later, follicular aspiration were performed by abdominal operation and the oocytecorona-cumulus complexes （OCCCs） were collected and trimmed under stereo microscope. The semen samples were obtained by means of masturbation 2-3 hours before operation. After centrifuged twice, the liquified semen was mixed with SW and incubated with 5％ CO₂ in air at 37℃ until GIFT.The OCCCs and 25μl processed semen were loaded into a special catheter and transferred into one fallopian tube. Then the transfer procedure was repeated for the other fallopian tube. The level of HCG in blood was examined from day 11 after transfer in order to diagnose pregnancy.更多还原