【摘要】 取出生后21～26天Sprague-Dawley未成熟雌性大鼠,分为实验与对照两组。实验组只/天注射己烯雌酚(DES)0.5mg(溶于芝麻油中),并按注射天数分为2、3、4、5天组;对照组注射等量的芝麻油。注射2天后,逐日取材固定,用光镜和电镜观察两组动物的卵巢和卵泡颗粒细胞。同时从各组卵巢中分离出卵泡颗粒细胞,用无血清McCoy 5a培养基培养,加入不同的激素,分为对照组、FSH组、DES+FSH组、FSH+hCG组。培养3天后,取材固定,用光镜、透射电镜、扫描电镜观察各组的细胞形态,结果为:1.实验组卵巢比对照组的大,卵泡也大。卵泡细胞增多,细胞体积大,分裂象多见。2.注射3天以上DES的卵巢中,闭锁卵泡和退化的卵泡细胞增多。3.电镜下,实验组卵泡细胞中与蛋白质合成有关的细胞器比对照组发达。4.体外培养的卵泡颗粒细胞,对照组细胞排列分散;FSH组细胞集聚成团,细胞表面微绒毛增多;细胞质内与合成蛋白质有关的细胞器比对照组的发达。DES+FSH组的细胞排列和结构与FSH组的相似。FSH+hCG组细胞体积增大,细胞表面微绒毛减少或消失。细胞质内出现滑面内质网,细胞开始黄体化。5.注射2～5天DES的各组细胞形态和结构均相似。从形态学来看,经DES处理的未成熟雌鼠的卵泡颗粒细胞的形态、排列和对不同激素作用下的变化,与文献报道的去垂体雌鼠的同类细胞相似。因此我们认为,未成熟雌鼠的卵泡颗粒细胞也能作为研究细胞分化的一种模型。更多还原

【Abstract】 Immature Sprague-Dawley rats (21-26 days old) were divided into an experimental group and a control group. The rats of the experimental group were subdivided according to the number of days of injection into 2, 3, 4, and 5 day groups. Each rat of the experimental group received an injection of DES 0.5 mg in sesame oil per day. Those of the control group received only the sesame oil. The animals were sacrificed 2-5 days after the injection. A light and electron microscopic study of the ovaries and granulosa cells were carried out. The granulosa cells from each experimental group were cultured in serum-free McCoy’s 5a medium by adding various hormones. The cultures were carried out in the following groups: control, FSH, DES+FSH, FSH+hCG. Three days after the culture, the morphology of cells of various groups were observed by LM, TEM and SEM. Results obtained were as follows:1. In the experimental group the ovaries and follicles were larger, the follicular cells increased in number, cell body was larger and more occurrence of cell divisions.2. Increase of atretic follicles and degenerating follicular cells in the DES group after more than three days of injection.3. Electron microscopy revealed that in the cytoplasm of follicular cells of experimental groups, organelles that participated in protein synthesis were better developed than those in the control.4. In vitro cells in the control group were dispersed; cells in the FSH group became aggregated, more microvilli appeared on the surface of these cells. In the cytoplasm, organelles that participated in protein synthesis were well developed than those in the control. The morphology of cells in DES+FSH group and FSH group were similar; cells in the FSH+hCG group increased in size, decrease or loss of the microvilli, SER appeared in the cytoplasm, and the cells became luteinized. 5. The morphology of cells in each different groups treated with DES from 2-5 days were similar.These results indicate that the morphology, arrangement, and changes in response to various hormones of the granulosa cells from DES-primed immature rats were similar to those cells from hypophysectomized rats. Therefore we suggest that granulosa cells of immature rats treated with DES can also be used as a model for studying cytodifferentiation.更多还原