【摘要】 用根癌土壤杆菌（Agrobacterium tumefaciens,At）酶的粗提取物加入到培养猪痢疾密螺旋体（Treponema hyodysenteriae,Th）的培养基中后接种Th(H₁₀菌株)在CO₂:H₂20: 80的环境中,37℃培养5天后,收获并制备Th抗原。这种抗原在微量凝集试验（microtitr-ation agglutination test, MAT）中能防止自凝现象。用这种Th H₁₀抗原检测了92头猪痢疾的血清和6头高免兔血清[其中用5株Th,1株无害螺旋体（Ti）高免)。结果表明Th与Ti高免血清之间有一定的交叉反应,但滴度是有显著区别的。48头SPF猪血清的效价均在64倍以下;而92头猪痢疾痢猪血清的抗体含量在128-2048倍之间,其中仅有一头猪的滴度为64倍,因此该法可用于猪痢疾病猪群的诊断。更多还原

【Abstract】 The crude extract of the Agrobacterium lumefaciens enzymes was added toculture medium for Treponema hyodysenteriae （Th） and the H₁₀ strain was cultured inCO₂:H₂=20:80 at 37℃ for 5 days. Then the Th antigen was havested and prepared.This antigen can prevent auto-agglutination phenomenon in microtitration agglutination test （MAT）.The sera from 92 swine dysentery （SD） pigs and 6 hyperimmune rabbits〔immunized with 5 strains of Th and 1 stain of treponema innocens （Ti）〕weredetected with the Th H₁₀ antigen. The titre between Th and Ti sera existed mildcross reaction, but it did not interfere with differentiation. The antibody titre of 48 SPFpigs and 92 SD pigs were≤64 and 128-2048 except 1 SD pig under 128, respectively.In conclution, the MAT method could be used for the diagnosis of SD herd.更多还原