【摘要】 用于分离和鉴定多杀巴氏杆菌的选择指示培养基的主要成份为胰蛋白膘(1%),胰蛋白胨(0.5%),蔗糖(1%),2.5%石蕊水溶液(0.25%)和1%中性红(0.5%)等。接种于该培养基后,于37℃培养24-48小时,多杀巴氏杆菌呈微红至玫瑰红色的露水珠样菌落,菌落边缘显示红色或兰绿荧光。而非多杀巴氏杆菌在37℃培养18-24小时的菌落显深紫红色(发酵蔗糖的细菌)或淡兰绿色或无色(不发酵蔗糖的细菌),在透射光线下观察,其边缘均不显荧光。当分离多杀巴氏杆菌时,如果在该琼脂平板培养基上出现3-5个典型菌落时,则对多杀巴氏杆菌的鉴别率通常可以达到100%。更多还原

【Abstract】 A selective indicator medium for isolation and identification of Pasteurellamultocida has been studied. The medium formula consists of tryptose (1%, W/V),tryptone (0. 5%. WIV), saccharose (1%, W/V), 2. 5%litmus water solution(0. 25%, V/V) and 1% neutral red solution (0. 5%, V/V) etc. After cultivation onthe plate agar at 37℃for 24-48 hours the colonies of pasteurella multocida appear asdewdrop-like and from slight red to rose in colour. The edge of the colonies show redor blue-green fluorescence. While the colonies of non-pasturelle multocida usually appeardeep purple-red or light blue-green or uncolored. Under the penetrating ray theiredges do not show fluorescent. On the isolation are found, if 3-5 typical Pasteurellamultocida colonies, its identification rates could be 100%.更多还原