【摘要】 用电子显微镜观察草鱼(Ctenopharyngodon idellus C.et v.) 出血病的病理切片,在病变细胞中发现有两种病毒颗粒,一种是呼肠孤病毒,以前已有报道;另一种为直径仅20—30nm的小病毒颗粒,系首次在鱼类细胞中发现。两种病毒主要侵染病鱼血管及微血管内皮细胞。内皮细胞受损而至解体,红细胞弥散细胞间隙,导致出血症状。此外还侵染肾上皮细胞,肾间质细胞、肠上皮细胞以及脾网状细胞、嗜酸性白细胞等。根据小病毒颗粒的形态及核酸分析,拟初步鉴别为小RNA病毒科病毒。更多还原

【Abstract】 Hemorrhage of Grass Carp is very disadvantageous to fisheries products, causing a mortality of 50-70% for 1 year old juvenile fish. The pathogen of hemorrhage of Grass Carp was studied in the early fifties. During 1978-1984, the Reovirus was isolated, which was named Grass Carp Reovirus (GCR).Observation under electron microscope of the tissues of kideny, spleen, liver, blood vessel, intestine and muscle of disease fish shows that there were two kinds of virosome in the pathological cells. The diameter of one particle was 80-100 nm and the other 20-30 nm. The main pathological (infested) cells contained endothelial cell of capillaries, reticuloendothelial cell, eosinophil leucocyte, pericyte and interstitial cell as well as other epithelioid cells. Since the endothelial cell of capillaries were damaged and lysed, hemorrhagic symptoms appeared in the tissue.According to the testing of viruses which were isolated and purified, the bigger one belonged to Reovirus while the other was temporarily defined as Picornaviridae.更多还原