【摘要】 实验对虾注射¹⁴C葡萄糖后,经过6天的饲养,然后用柱层析法分离对虾蛋白氨基酸,用液体闪烁计数器测定每一种氨基酸的放射性。结果发现:对虾蛋白中的半胱氨酸（cys）、天冬氨酸（Asp）、丝氨酸（Ser）、谷氨酸（Glu）、脯氨酸（Pro）和丙氨酸（Ala）都有明显的放射性,表明上述几种氨基酸是中国对虾自身能够合成的非必需氨基酸;而苏氨酸（Thr）、甘氨酸;（Gly）、缬氨酸（Val）、蛋氨酸（Met）、异亮氨酸（I1e）、亮氨酸（Leu）、酪氨酸（Tyr）、苯丙氨酸（Phe）、赖氨酸（Lys）、组氨酸（His）、精氨酸（Arg）和色氨酸（Trp）,都没有放射性标记,说明这些氨基酸是中国对虾不能从葡萄糖来合成的,其中除酪氨酸被认为可能从苯丙氨酸来合成和甘氨酸尚需进一步验证外,其它氨基酸对于中国对虾的生长可能都是必需的。更多还原

【Abstract】 The problem of the essential amino acids of the prawn, Penaeus orientalis, which is important in preparing its diet and in studying its; nutritional physiology, was investigated by means of radiometric method using (U14C) glucose. After injecting (U-14C) glucose, the prawns were reared for six days and then the constituent amino acids of its proteins were isolated by column chromatography and the radioactivity of individual amirio acids was measured by liquid scintillation counter.The injection of (U-14C) glucose into: prawns resulted in the labeling of the amirio acids cysteine, aspartic acid, serine, glutamic acid, proline, alanine. These amino acids were supposed to be nonessential for the prawn. Threonine, glycine, Valine, methionirie, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine, tryptophan, incorporated no sig-nificant radioactivity from (U-14C) glucose. These amino, acids except tyrosine being thought to be nonessential if phenylalanine was supplied and glycine needing to be further tested and Verified, were inferred not to be synthesized, or to be synthesized insufficiently, de novo and were probably essential for the growth of the prawn.更多还原