【摘要】 作者将测定大肠杆菌液β-萄萄糖醛酸酶（β-G）活性的方法加以改进。预试发现:菌液的β-G活性受细菌培养基的形式、破碎细胞壁的方式、酶反应保温时间、细菌培养时间及细菌致溶剂加量等因素的影响。选用L₁₆（4³×2⁶）正交试验表,筛选出测定大肠杆菌 3360(O₁₅₇K₈₈)菌液中β-G活性的最优条件为:细菌在斜面培养基上37℃培养48小时,收集菌液经-70℃冷冻过夜,升温至37℃ 融化,再滴加细胞致溶剂8.3％Triton X-100溶液 2～8滴使菌破壁,测定β-G活性时酶反应保温时间为48小时。更多还原

【Abstract】 There is definite link between theformation of the bilirubin gallstones andβ-glucuronidase produced by bacteria whichinvolve decomposing combined bilirubininto uncombined bilirubin. The optimum for determination of β-glucuronidase activity in the fluid of E. coli3360 strain (O₁₅₇K₈₈) was selected by L₁₆（4³×2⁶） orthogonal experiment, i. e.,the cultures were incubated on solid medi-um at 37℃, 48 hours; the fluid growth ofbacteria was collected, frozen at -70℃through night, and melted at 37℃, thenadded 2 to 8 drops of 8.3% Triton X-100;reaction of the enzyme shoud be incu-bated 48 hours when the activity of β-glu-curonidase was determined.更多还原