【摘要】 作者采用胆酸钠透析法对细菌(Pseudomonas maltophilia)分泌的HCG受体蛋白与脂质体进行了重组,经电镜观察和测定重组前后受体的结合曲线,表明脂质体形成并与受体重组,且使受体活性增加。此外,作者还测定了磷脂与蛋白质的比例、离子强度以及pH对重组的影响。更多还原

【Abstract】 The HCG receptors extracted and puri-fied from Pseudomonas maltophilia was re-constituted with liposomes by means ofsodium cholate dialysis. Soybean phospoli-pid was used to form liposomes for thereconstitution with the receptor. The re-constituted liposomes and unreconstitutedreceptor were separated through 43000r/mincentrifugation. The observation under elec-tron microscope showed that the receptor hadbeen reconstituted to liposomes.By the dis-placement of the binding of the reconstitutedand pre-reconstituted receptor it was alsoindicated that the binding activity of thereceptor increased after reconstitution. Wecompared the influence of several conditionson the result of reconstitution. We foundthat the reconstitution result was related tothe rate of mol content fo protein and phos-pholipid:the smaller it was, the better theresult was. According to our experiment,low pH was not favourable for reconstitutionbecause of the large amount of acidicphospholipid contained in soybeanphospholipid and the white precipitation ofphospholipid produced in acid condition.Neither high nor low ionic concentrationwas advantageous for the reconstitution.Hence, we can draw a conclusion that thereconstitution is related to the interactionof ions. But further research needs to beconducted.更多还原