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钩端螺旋体外膜免疫生物学特性的研究

STUDIES ON IMMUNOBIOLOGICAL PROPERTIES OF OUTER ENVELOPE OF LEPTOSPIRES

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【作者】 钟为民戴保民王远萍杨志梅

【Author】 Zhong Weimin;Dai Baomin Research Unit of Leptospirosis Wang Yuanping;Yang Zhimei Electron Microscopy Laboratory

【机构】 华西医大钩端螺旋体病研究室华西医大电子显微镜室华西医大电子显微镜室

【摘要】 作者采用改良钩体外腹提取法,提取五株钩体外膜均获成功。电镜观察发现五株钩体外膜超微结构层数存在明显差别。豚鼠保护力试验证实赖型017株外膜对同型强毒株攻击具有明显的保护作用。SDS-PAGE和免疫印迹分析表明017株外膜蛋白具有良好的抗原性。017株和601株外膜蛋白电泳图谱及兔抗血清交叉识别图谱虽非常相似,但存在明显差别。

【Abstract】 The modified method for preparing theouter envelope of leptospires was used suc-cessfully for the isolation of the outer en-velopes of five leptospiral strains (L.inter-rogans serovar Lai strain 017 and 601, L.heb-domadis strain 245, L.biflexa strain patoc Ⅰand Leptonema illini strain 3055). With thetransmission electron microscopy, obviousdifferences were noted in the number ofmembrane-layer or the isolated outer enve-lopes. The immunological protective testconfirmed that the outer envelope of scrovarLai strain o17 had significant protective po-tency against challenge of the homologoushigh virulent strain.SDS-PAGE and immu-noblotting analysis demonstrated that itsproteins possessed antigenicity. The outerenvelope protein profiles and cross-recogni-zation by rabbit antisera were very similarbetween strain o17 and strain 601, but someobvious differences were found simultan-eously.

【基金】 国家自然科学基金
  • 【被引频次】9
  • 【下载频次】14
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