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亲和素-生物素酶复合物酶联免疫吸附技术应用于检测HBsAg的研究

THE USE OF AVIDIN-BIOTIN PEROXIDASE COMPLEXELISA FOR THE EXAMINATION OF HBsAg

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【作者】 殷强仲叶梅君许欣刘衡川刘军刘相民

【Author】 Yin Qiangzhong;Ye Mei jun;Xu Xin;Liu Hengchuan Liu Jun;Liu Xiangmin Department of Medical Technology

【机构】 华西医大医学检测教研室华西医大医学检测教研室

【摘要】 作者应用免疫化学 ABC-ELISA 作人群血清中 HBsAg 的检测,验证了该法在检测 HBsAg 方面具有高敏感性。ABC-ELISA 与 ELISA、R-PHA 及 RICEPA 三种方法相比,HBsAg 的检出率按其敏感程度高低为 ABC-ELISA>ELISA>R-PHA>RICEPA。ABC-ELISA 与经典 ELISA单独相比,其HBsAg 检出率由 ELISA的16.07%提高到 ABC-ELISA 的23.58%,并且可将ELISA漏检的32例 HBsAg 携带者检出。此外,该法在检测 HBsAg中还具有特异性高,操作简便和快速等优点。

【Abstract】 A new serological method: Avidin-Biotin peroxidase complex-ELISA (ABC-ELISA) was used in the detection of HBsAgin the serum of a population. The serumsamples were tested by the ABC-ELISA,ELISA, RPHA and RICEPA methods. Thesensitivity of the ABC-ELISA method ascompared with the other three methods isin the order of ABC-ELISA ELISA,R-PHA,RICEPA. When ABC-ELISA was used instead ofELISA, the results showed that the detec-tion rates of HBsAg raised from 16.07% to23.58%. Based on these experiments, ithas been demonstrated that ABC-ELISA isa very sensitive, highly specific and rapid(3h), method for the detection of HBsAgand these results also indicated that thismethod may be used for epidemiologicalsurvey.

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