【摘要】 作者应用免疫化学 ABC-ELISA 作人群血清中 HBsAg 的检测,验证了该法在检测 HBsAg 方面具有高敏感性。ABC-ELISA 与 ELISA、R-PHA 及 RICEPA 三种方法相比,HBsAg 的检出率按其敏感程度高低为 ABC-ELISA>ELISA>R-PHA>RICEPA。ABC-ELISA 与经典 ELISA单独相比,其HBsAg 检出率由 ELISA的16.07％提高到 ABC-ELISA 的23.58％,并且可将ELISA漏检的32例 HBsAg 携带者检出。此外,该法在检测 HBsAg中还具有特异性高,操作简便和快速等优点。更多还原

【Abstract】 A new serological method: Avidin-Biotin peroxidase complex-ELISA (ABC-ELISA) was used in the detection of HBsAgin the serum of a population. The serumsamples were tested by the ABC-ELISA,ELISA, RPHA and RICEPA methods. Thesensitivity of the ABC-ELISA method ascompared with the other three methods isin the order of ABC-ELISA ELISA,R-PHA,RICEPA. When ABC-ELISA was used instead ofELISA, the results showed that the detec-tion rates of HBsAg raised from 16.07% to23.58%. Based on these experiments, ithas been demonstrated that ABC-ELISA isa very sensitive, highly specific and rapid(3h), method for the detection of HBsAgand these results also indicated that thismethod may be used for epidemiologicalsurvey.更多还原