【摘要】 作者制备了麦芽假单胞菌丙酮粉末,用以纯化该菌的HCG结合物质,并比较了不同溶剂对结合物质的增溶效果,按1％去氧胆酸钠 1ml加40mg麦芽假单胞菌丙酮粉末的比例,振荡抽提30分钟,效果最佳。更多还原

【Abstract】 It has been documented that the P.mal-tophilia was capale of binding ¹²⁵I-labeledHCG with high affinity.In order to furtherthe study of the nature of the HCGbinding substances, efforts were made toisolate them. We prepared the bacteriaacetone powder that had the HCG bindingactivity. P.maltophilia acetone powder wasextracted with different solvents （distilledwater, 0.1 M acetic acid, 0.1 M ammoniumhydroxide, phosphate buffered saline, 1%triton X-100 and 1% sodium deoxycholate）for 30 min at 4℃. The extract was thencentrifuged （30000g, 30 min）. The clearsupernants were precipitated with 8O% inthe pressence of 5mM benzamidine. Effi-cient extraction could be achieved with 1%sodium deoxycholate（76.8%）,when the ratioof bacteria acetone powder to solvent was40 mg to 1 ml.The 1% sodium deoxycholatewas the best solvent for the extraction.更多还原