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麦芽假单胞菌的HCG结合物质的提取

A STUDY OF EXTRACTION OF HCG BINDING SUBSTANCES FROM PSEUDOMONAS MALTOPHILIA

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【作者】 陈曼玲邱明均蓝天鹤夏敏

【Author】 Chen Manling;Xia Min;Qiu Mingjun;Lan Tianhe Department of Biochemistry, Molecular Biology Laboratory

【机构】 华西医科大学生物化学教研室华西医科大学分子生物学实验室

【摘要】 作者制备了麦芽假单胞菌丙酮粉末,用以纯化该菌的HCG结合物质,并比较了不同溶剂对结合物质的增溶效果,按1%去氧胆酸钠 1ml加40mg麦芽假单胞菌丙酮粉末的比例,振荡抽提30分钟,效果最佳。

【Abstract】 It has been documented that the P.mal-tophilia was capale of binding 125I-labeledHCG with high affinity.In order to furtherthe study of the nature of the HCGbinding substances, efforts were made toisolate them. We prepared the bacteriaacetone powder that had the HCG bindingactivity. P.maltophilia acetone powder wasextracted with different solvents (distilledwater, 0.1 M acetic acid, 0.1 M ammoniumhydroxide, phosphate buffered saline, 1%triton X-100 and 1% sodium deoxycholate)for 30 min at 4℃. The extract was thencentrifuged (30000g, 30 min). The clearsupernants were precipitated with 8O% inthe pressence of 5mM benzamidine. Effi-cient extraction could be achieved with 1%sodium deoxycholate(76.8%),when the ratioof bacteria acetone powder to solvent was40 mg to 1 ml.The 1% sodium deoxycholatewas the best solvent for the extraction.

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