【摘要】 本文报告应用选择性培养基和厌氧手套箱系统,研究与国内青少年牙周炎(JP)病人有关的革兰氏阴性茵群。对照组5个健康青年人龈下菌斑的培养结果显示:龈下茵斑内细菌量少,细菌种类也较少。10个JP病人14个疾病部位的研究结果表明:疾病区细菌量多,种类复杂。优势菌群主要为口类杆菌、产黑色素类杆菌和核粒梭杆菌。检出率和比例均远远高于其他种类的细菌。本研究无一例检出放线共生嗜血杆菌,提示该菌不是国内JP病人的主要致病菌。更多还原

【Abstract】 Predominant cultivable studies of the subgingival plaque from juvenile periodontitis(JP)show that JP is associated with gram negative bacteria,especially with Haemoph-illus actinomycetemcomitans and Capnocytophaga and other species.This study was desi- gned to study these species with chinese JP patients using selective cultivable techniquein anaerobic glove box system.The media used include MGB,containing malchite green8μg/ml and bacitracin 128μg/ml to grow H.actinomycetemcomitans,KV,containing kana-mycin 75μg/ml and vancomycin 10μg/ml to support Bacteroides,and FS,containing crystalviolet 7μg/ml,neomycin 30μg/ml and vancomycin 10μg/ml to allow Fusobacterium growth.Five normal gingival sulcus samples from health young people show that bacterial am-ount of subgingival plaque was not much and there were less kinds of bacterial species.Fourteen subgingival plaque samples from 10 JP patients demonstrate that there ismuch amount of bacteria and more complex kinds of bacterial species.The predominantspecies are Bacteroides,espeeially B.oris and B.melaninogenicus,and Fusobacterium nuc-leatum.The frequency of detection(expressed as percent of sites)of these species are 50,50,and 71.4,respectively.The mean percentages of various species are 7.88,9.40,and 4.40,respectively.Both are quite higher than other species.H.actinomycetemcomitans was not detected in all cases.This appears that H.actinomy-cetemcomitans is not the majour pathogen of China JP patients and there is no the singleor simple specific pathogen for JP.更多还原