【摘要】 链毒素——牛血清白蛋白(ST—BSA)致敏豚鼠并攻击的过敏反应率为88%,休克率为76%,死亡率为44%,对照组动物则活动如常:致敏组豚鼠离体回肠经抗原攻击后紧张度升高、蠕动加强,反应率为100%,对照组均为阴性反应;致敏组肥大细胞脱颗粒率为72%,对照组为36%,PCA试验证明,致敏组阳性反应率100%,抗体滴度为1:256,抗血清56C灭活1h阳性反应率仍为100%。但滴度为1:100。更多还原

【Abstract】 ST-BSA was synthesized from streptomycin and bovine serum albumin.Guinea-pigs could be sensitized with ST-BSA. The anaphylactic rate was 88%,and the anaphylactic shock rate was 76% and the death rate was 44% but the rateof anaphylactic reaction in the control group was 0.The test in which the isola-ted ileum of the sensitized guinea pigs released anaphylactic mediators whed challenged with ST-BSA demonstrated that the tension and peristalsis of the isolated ileum were intensified. The rate of positive reaction was 100%, and this reactioncould be blocked pertly by benadryl. The incidence of positive reaction in the co-ntrol group was 0. The test of degranulation of mast cells showed that the rateof degranulation in the testing group was 72%, and that in the control group was36%. The PCA tests of heterogeneous animals demonst rated that the rate of po-sitive reaction was 100%, the titer was 1: 256, whereas the rate of positive re-action in the control group was 0. When the antiserum was heated at 56℃ for 1hour, the rate of positive reaction was still 100% and the titer was 1:100. Theresults showed that ST-BSA was one of the sensitinogens, and that there isclose relationship between ST-BSA and an aphylactic shock. It is important toeliminate the impurities in streptomycin to decrease the allergy due of streptomycin.更多还原