【摘要】 介绍同时测定脑脊液中单胺类遂质及代谢产物5-HT,5-HTP,Trp,MHPG,HVA和5-HIAA的高效液相色谱法。50μ脑脊液样品,不需预处理,20分钟可完成6种物质的分析。本文还讨论了室温、流动相pH值、检测器检测条件等因素对分离及检测的影响。更多还原

【Abstract】 Simultaneous determination of CSF monoamine metabolites MHPG, HVA, 5-HIAA,5-HTP and tryptophan by a HPLC method with electrochemical and ultraviolet detectors is introduced.No pretreatment of sample was neccessory, and it took about only 20 min. to run a sample. The effect of pH and temperature resolution on capacity was examined.The electrochemical response was influenced by pH of the mobile phase and oxidation potential of the detector.The concentrations of these substances obtained were identical with previous litetatures.更多还原