【摘要】 在正交偏光显微镜下本文观察到恶性淋巴瘤细胞有较明显的双折射现象,表明恶性淋巴瘤细胞的生长伴随有液晶态及特征性的生物聚合体结晶过程。实验中还发现正常淋巴细胞、经PHA刺激的淋巴细胞以及恶性淋巴细胞引起偏振光干涉的强度明显不同,其差异表明这三种细胞处于不同的液晶状态,因此,这种差异性有可能作为肿瘤早期诊断的一种指标。更多还原

【Abstract】 Some optical properties of Burkitt’s lymphoma cell lines and normal lymphocytes were studied by using polarized light microscopy. The cells derived from Burkitt’s lymphoma (P3HR-1, Raji, Ramos and BJAB)and transformed by Epstein-Barr virus (B95-8) present obviously birefringence phenomena. A strong bright ring corresponding to the cell membrane and some bright areas within the cells were shown under the polarized light. It was suggested that there were some liquid crystals and crystallizing processes for characteristic biological aggregation accompanying the growth of malignant tumor cells. In addition, the lymphocytes treated with PHA were also appearing a weak bright ring and bright areas. The phenomena illustrated that the liquid crystal state was changed when the lymphocytes were stimulated. However, there were no intracellular bright areas to be observed in the normal lymphocytes, but only a dim ring. The results obtained in these experiments implied that there were some differences of liquid crystal states among the three kinds of cells. Whether the phenomena may be recommended as one of the parameters used for early clinical diagnosis of cancer is an interesting subject.更多还原