【摘要】 以妊娠兔肝细胞膜碎片为受体制剂,用乳过氧化物酶法或Iodogen法碘化标记人生长激素,建立生长激素放射受体分析法。本方法灵敏度为0.67±0.11ng/ml。用RRA和RIA两种方法同时对72例正常成人、102例肢端肥大症患者测定血清GH浓度,结果表明正常成人两种方法测定结果无显著差异(P>0.05),而肢端肥大症患者的RRA值<RIA值(术前P<0.01,术后P<0.05)。更多还原

【Abstract】 A radioreceptor assay for human growth hormone was developed. The receptor preparation was 25,000g pellet from the livers of pregnant rabbits. lodination of GH with 125I was preformed by the methods of Lactoperoxidase and Iodogen. The sensitivity of assay was 0.67±0.11 ng/ml serum.Serum hGH levels in 72 cases of normal subjects, 102 cases of acromegaly were mear sured by RRA, and the results were compared with those obtained by RIA.更多还原