【摘要】 为了搞清在海豚肝脏中存在的含有高浓度硒和汞的新化合的的存在形态及化学性质,本文采用了丙酮脱脂、无机盐溶液提取、限外过滤及离子交换、凝胶色谱、纸色谱、高速液相色谱等各种色谱分离法,对于海豚肝脏中含有硒和汞的新化合物进行了抽出、分离、纯化和精制。并比较了各种色谱法的纯化和精制的效果,制定了最佳的提纯操作方案。通过本实验,确证了在海豚肝脏中存在摩尔分子比1:1的硒和汞的新化合物,并探讨了这个化合物的存在状态及化学性质。更多还原

【Abstract】 It is well known that dolphins sometimes accumulate Hg and Se as high as 100μg/g or more in their livers. In the present study, compounds containing Hg and Se in a dolphin liver were extracted and purified by cation exchange anion exchange, gel filtration, paper and liquid chromatographies. The Hg and Se contents in every fraction were determined with atomic absorption spectrometry by using the palladium addition method. In all chromatographies Hg and Se appeared in the same fractions in a 1:1 molar ratio. The purified compound is water-soluble with molecular weight of less than 1000, containing aminogroups as well as Hg and Se at a 1:1 molar ratio.更多还原