【摘要】 高温用于恶性肿瘤的治疗是近年来颇受重视的工作,但高温处理不可避免地对肿瘤组织周围正常细胞也将产生影响。为了研究高温对正常细胞的影响,本文以人红细胞为实验模型,研究其细胞热损伤的机制。红细胞经不同温度处理二十分钟后,通过扫描电镜和生化分析方法探讨红细胞的形态变化,溶血作用和膜结合酶-乙酰胆碱酯酶、Na·K·Mg-ATP酶活性的变化。结果表明,在本实验条件下细胞形态的改变对高温最敏感:48℃高温处理后,绝大部分细胞变形,50℃保温使溶血显著增加,膜结合酶热变性温度则超过54℃。更多还原

【Abstract】 Over the past decade there has been a great interest in the use of hyperthermia to treat cancer. The action of hyperthermia on normal tissue is worthy of mention during heating treatment. In order to study the effect of hyperthermia on normal cell, human erythrocyte is a convenient model for the investigation of cell thermal damage. Erythrocyte samples were incubated at various temperatures for 20 min. This study showed that the erythrocyte deformation to hyperthermia occured more susceptibly than Na. K. Mg-ATPase and ethylcholinestase thermal denaturation under our experimental condition. The dramatic increase in erythrocyte deformation and heamolysis in pBS occurred at temperatures between 48°C to 50°C. The membrane enzyme thermal denaturation occurred at temperatures in excess of 54°C.更多还原