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缺氧、缺糖对培养心肌细胞形态和α-羟丁酸脱氢酶活性的影响

The Effect of Hypoxia and Hypoglucose on the HBDH Activity of Cultured Myocardial Cells

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【作者】 张友云; 陈锡昌; 朱世柱; 冯晓祥;

【Author】 Zhang Youyun, et al (Department of Human Anatomy, Hubei Medical College, Wuhan, China)

【机构】 湖北医学院解剖学教研室; 湖北医学院解剖学教研室;

【摘要】 本实验在缺糖伴或不伴缺氧条件下,均可引起早期培养心肌细胞出现明显细胞损伤性反应,表现为细胞退行性变、细胞自律性搏动减慢以及心肌细胞释放α-羟丁酸脱氢酶增加。培养液中α-羟丁酸脱氢酶活性增加,是早期培养的心肌细胞损伤性反应敏感而稳定的指标,它为监护心肌缺血的动态变化和疗效观察提供了一种有价值的实验依据。

【Abstract】 The results presented here indicated that the cultured early myocardial cells of infant rat showed obviously the reaction of the cellular damage under the condition of the hypoglucose with hypoxia and the hypoglucose with oxygen. The mark of the cellular damage was an excessive relaese of HBDH. The changes of activity of this enzyme were a sensitive and stable index of injuring reaction of the myocardial cells, and it provided a significant experimental evidence for supervising the changes of myocardial infarct and observing curative effect.

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