石家庄入冬到初春三年中气温年度之间的月平均和月最低温度,没有异常变化。通过调查1986—1987年度绞股兰越冬期间土壤耕层5、10、15、20、25厘米处温度,代表了石家庄历年来土壤耕作层的温度变化规律,此温度适合由山东省引入的日本产绞股兰生长,冬季田间不用采取特殊措施就可安全自然越冬。以绞股兰茎切段为材料,采用N_6和M_s培养基均能诱导获得无菌苗;继代繁殖中,N_6培养基较M_s培养基更佳,最适激素配比为B A 0.8mg/L、IAA0.5mg/L;生根为N_6培养基附加激动素KTO.5mg/L、IAA0.1 mg/L;移栽中选用山地草甸土或本地区一般土壤基质,均能使移栽后的试管苗安全成活。应用常规定性,薄层色谱和定量分析绞股兰样品表明,绞股兰材料含有与部分人参皂甙相同的绞股兰皂甙,且材料经过组培繁殖后其成分不变;采用水和70%乙醇溶剂提取绞股兰皂甙,均较甲醇提取得率高。
【英文摘要】
At Shijiazhuang in Hebei province, the average month ambient temperature and minimum ambient temperature were no abnormal change from the early winter to to early spring in the last three years. By investigating the temperature of the places in the 5,10,15,20 and 25cm depth of the top soil in the winter of 1986-1987, it was found that basically it reflected the law of the temperature change in the top soil over the years in this area .This experiment shows that the Japanese Gynostemma pentaphyllum Makino tr...