【摘要】 本文研究了酸性介质中,磷钼酸盐可与罗丹明6G形成络合物,从而淬灭罗丹明6G的荧光,提出并研究了利用上述过程测定水中微量磷的方法,试验了测定过程的各种条件:激发和发射光谱波长、试剂浓度、体系的酸度等。发现该方法简便、灵敏、准确。更多还原

【Abstract】 A simple method for determination of phosphorus in natural water by using fluorescence quenching of rhodamine 6G with molybdophosphate is described. The fluorescence intensity of rhodamine 6G was diminished by molybdophosphate which was formed by reaction between orthophosphate and molybdaten hydrochloric acid medium. The diminished fluorescence intensity of rhodamine 6G waspropotional to the concentration of phosphorus in aq. solution (at Ex 350nm, EM 550nm). The colibration curve covered 0—10 ppb of phosphorus. The relative standard variation of 6 determinations of 5 ppb phosphorus was 9% and the addition recoveries of 3—5 ppb spiked phosphorus were 96—107%. Several foreign ions were tested and no interferences were observed. The present method is sensitive, accurate and easy to operate. It can be used for direct determination of phosphorus in natural water without preseparation.更多还原