【摘要】 应用扫描电镜,结合选择性细胞抽提技术制备核骨架—Lamina—中间纤维系统的方法,对牛肾继代组培细胞进行了观察。发现牛肾细胞中间纤维交织成密集的网络,遍布整个细胞质,并且从三维空间上清晰地显示了中间纤维与核膜下蛋白质性质的Lamina连结的关系。同时观察到核骨架纤维有若干根单纤维融合成粗大纤维的现象,并且核骨架纤维也与Lamina相连。文中对中间纤维可能的功能特别是其骨架功能,核骨架、Lamina、中间纤维的关系,以及使用扫描电镜观察这些结构的优点进行了讨论。还对牛传染性鼻气管炎病毒感染后的细胞的核骨架纤维做了初步观察。更多还原

【Abstract】 We had employed scanning electron microscopy together with selective extraction methodto prepare the nuclear matrix (NM)-lamina-intermediate filament (IF) system. We had obser-ved the NM-lamina-IF of bovine kidney cell cultures, and found that the IFs formed a thicknetwork spreading over whole cell. The connection of IFs with nuclear lamina was clearlyshown in three-dimensional. Besides monofilaments, there are also thicker filaments whichmay be formed by several monofilaments in the nuclear matrix. We had discussed the func-tions especially its skeletal function and the relationship of NM, lamina, IF; and the merit ofobservation on this system by the means of SEM. The NM in the cell infected with infectiousbovine rhinotracheitis virus (IBRV) was observed preliminarily.更多还原