【摘要】 从正常生育期人子宫肌肉组织分离、纯化了神经节苷脂（Gls）和中性鞘糖脂（N-GSL）,测定了两类鞘糖脂的含量、对其组分进行 HPTLC 谱分析,并通过酶水解法对 Gls 主要成份作了鉴定。结果表明子宫肌肉组织的 Gls中 GD₃含量高达56. 6%,其次为 GM₃占16. 9%,GT_1b占9. 8%。此外,还有少量 GM₁、 GD_1a、与 GD_1b。N-GSL 主要组分为 CTH 和 CQH,各为30. 5%与35. 4%,次为 CDH 占16. 8%,CMH 与含多糖基（≥5个糖基）的组分各<10%。本样品每100克湿重神经节苷脂含量约3. 48mg（以唾液酸表示）,N-GSL 为3. 71mg（以鞘氨醇表示）。更多还原

【Abstract】 Gangliosides（Gls）and neutral glycosphingolipids（NGSL）were isolated and purified from human myometrium by DEAE-Celluloseand Iatrobeads column chromatography.The contents of Gls and NGSLwere determined by spectrophotometry,and the compositions were ana-lyzed by HPTLC.The major Gls component GD₃,which was purifiedby salt gradient elution from ion exchange column,was identified byenzymatic hydrolysis.GD₃ content was found to be the highest（56. 6%of total sialie acid）,while those of GM₃ and GT_1b were 16. 9% and9. 8%,respectively.Other Gls’s were GM₁,GD_1a and GD_1b,（about 5%each）.The major components of NGSL were CTH and CQH,（30. 5%and 35. 4%）,then CDH（16. 8%）;CMH and polysaccharide containingcomponents were less than 10% each.The contents of total LBSA and NGSL were 3. 48mg and 3. 71mg per100 grams of wet weight tissus.更多还原