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鼠疫FI单克隆抗体致敏红细胞用于反向血凝抑制试验检测鼠疫FI抗体的试验研究

DETECTING FI ANTIBODY OF Y.PESTIS BY COUNTER HEMAGGLUTINATION INHIBITION TEST WITH FI McAb SENSITIZED RED CELL

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【作者】 徐秉臣韩松杨林

【Author】 Xu Bing-chen, et al (Xinjiang Institute for Endemic Disease Control and Research, Urumqi)

【机构】 新疆地方病防治研究所新疆地方病防治研究所 乌鲁木齐乌鲁木齐乌鲁木齐

【摘要】 本文介绍了鼠疫FI MCAb致敏红细胞用于反向血凝抑制试验检测鼠疫FI抗体的试验。结果表明,用该法检测95份正常人和8种动物血清,7种非鼠疫菌免疫动物血清,全部阴性;检测9份抗鼠疫强毒株和1份弱毒株血清及1份接种鼠疫菌苗的人血清,全部阳性,滴度比间接血凝试验(IHA)高2~1~2~3。该法可省去另做一排抑制试验的操作程序,较IHA简便、快速,在鼠疫疫源地调查和疫情监测中有良好的应用前景。

【Abstract】 FI McAb sensitized red cells were used in the counter hemagglutination inhibition test to detect FI antibody of Y, pestis. All the tested sera from 95 normal persons, 8 species o"f animals and 7 animals immunized with non - plague bacterin showed negative results while positive results were obtained from all the tests, on 9 antipest sera against strong virulent Y.pestis, 1 serum against weak virulent Y. pestis and 1 human serum from a subject inoculated with plague bacterin. The titres showed by the present method were much higher than those by IHA, it proved a simpler and faster method without the trouble to prepare an aditional inhibition test. The present method has, therefore, a promising future in the epidemiologic surveilance of plague foci.

  • 【文献出处】 地方病通报 ,Endemic Disease Bulletin , 编辑部邮箱 ,1988年02期
  • 【被引频次】3
  • 【下载频次】27
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