【摘要】 应用杂交瘤技术制备出胃癌单克隆抗体(G8511)用间接免疫组化法染色,91例胃癌中59例呈阳性反应,对109例其它内脏的恶性肿瘤染色,阳性结果仅见于食道癌(2/13)、十二指肠癌(2/4)及肝癌(1/8),其余84例36种恶性瘤均为阴性。8例肠化生中,5例不完全型及1例混合型为阳性,两例完全型为阴性。15例胃粘膜异型增生中,9例阳性。结果表明,G8511是胃癌相关抗原的抗体,此抗原亦见于部分癌前病变。更多还原

【Abstract】 A monoclonal antibody against gastric carcinoma (G8511) was produced by hybridoma technique. Spleen celis of BALB/C mice immunized with gastric cancer antigen were fused with murine myeloma cell line NS-1. Using G8511 for indirect immunohistochemical staining of paraffin seclions, 59 out of 91 cases (65%) of gastric carcinoma showed positive reaction. Among the different types of gastric carcinoma, mucinous cell carcinoma had the highest positive rate of 87%(13/15). When 109 cases of malignant tumors of other organs were screened by the same technique, positive cases were found among cancers of esophagus (2/13), duodenum (2/4)and liver (1/8), while 36 types (84 cases) of malignant tumors of other organs were negative. In the 8 cases of intestinal metaplasia (IM), positive reaction was seen in 5 cases of imcomplete type and one case of mixed type of IM, while 2 cases of complete IM shovved negative reaction. Positive reactioa was also detected in 9 out of 15 cases of gastric mucosal dysplasia. The above results indicate that G8511 is an antibody against gastric cancera-ssociated antigen.更多还原