【摘要】 采用改良的放射受体测试技术,建立了一种灵敏、稳定、快速、精确的血清1,25-（OH）₂-D₃（1,25—Dihydoxyvitamin D₃,Dihydroxycholecalciferol,DHCC）检测方法,并应用于先天性佝偻病及青春期佝偻病的临床研究,佝偻病观察组血清DHCC含量显著低于正常对照组,且对佝偻病的检出率显著高于低钙征、血生化检查及X线检查,故血清DHCC检测可作为佝偻病灵敏而特异的早期诊断指标。基于新生儿血清DHCC含量与其母体紧密相关,检测孕妇血清DHCC含量,可作为诊断、防治先天性佝偻病的重要依据。更多还原

【Abstract】 A modifed radioreceptor assay for 1,25-(OH)2-D3 (1,25-Dihydroxy vitamin D3, Dihydroxycholecalciferol, DHCC) in serum was established, which was proved to have high sensitivity, stability and accuracy, and wasj applied in patienss with rachitis. The results showed that DHCC level in serum in rachictic patients was lower than of the control.The detection rate of DHCC was obviously higher than that through symptoms, liboratory tests and X-ray examination in patients with rachitis .Therefore, this assay technique can be used as a sensitive indicator for diagnosis of rachitis.It was also found that DHCC level in mothers was correlative with their infants, so that assay for DHCC level in pregnant mothers has significance in prevention and treatment of rachitis in their infants.更多还原